Laboratory evolution of a biotin-requiring Saccharomyces cerevisiae strain for full biotin prototrophy and identification of causal mutations
JM Bracher, E de Hulster, CC Koster… - Applied and …, 2017 - Am Soc Microbiol
Biotin prototrophy is a rare, incompletely understood, and industrially relevant characteristic
of Saccharomyces cerevisiae strains. The genome of the haploid laboratory strain CEN.
PK113-7D contains a full complement of biotin biosynthesis genes, but its growth in biotin-
free synthetic medium is extremely slow (specific growth rate [μ]≈ 0.01 h− 1). Four
independent evolution experiments in repeated batch cultures and accelerostats yielded
strains whose growth rates (μ≤ 0.36 h− 1) in biotin-free and biotin-supplemented media …
of Saccharomyces cerevisiae strains. The genome of the haploid laboratory strain CEN.
PK113-7D contains a full complement of biotin biosynthesis genes, but its growth in biotin-
free synthetic medium is extremely slow (specific growth rate [μ]≈ 0.01 h− 1). Four
independent evolution experiments in repeated batch cultures and accelerostats yielded
strains whose growth rates (μ≤ 0.36 h− 1) in biotin-free and biotin-supplemented media …
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